Carnitine biosynthesis. Hydroxylation of N6-trimethyl-lysine to 3-hydroxy-N6-trimethyl-lysine

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Biosynthesis of carnitine and 4-N-trimethylaminobutyrate from 6-N-trimethyl-lysine.

The conversion of 6-N-[Me-(14)C]trimethyl-lysine into carnitine and 4-N-trimethylaminobutyrate (butyrobetaine) was demonstrated in rats kept on a lysine-deficient diet. After the rats were given [(14)C]trimethyl-lysine for 4 days, a total of 17% of the injected label was recovered as carnitine from carcass and urine extracts. Another 8% of the trimethyl-lysine label was converted into 4-N-trime...

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Lysine: N6-hydroxylase: stability and interaction with ligands.

Recombinant lysine:N6-hydroxylase, rIucD, which is isolated as an apoenzyme, requires FAD and NADPH for its catalytic function. rIucD preparations have been found to undergo time-dependent loss in monooxygenase function due to aggregation from the initial tetrameric state to a polytetrameric form(s), a process which is reversible by treatment with thiols. Ligand-induced conformational changes i...

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Biosynthesis of carnitine and 4-N-trimethylaminobutyrate from lysine.

The conversion of l-[U-(14)C]lysine into carnitine was demonstrated in normal, choline-deficient and lysine-deficient rats. In other experiments in vivo radioactivity from l-[4,5-(3)H]lysine and dl-[6-(14)C]lysine was incorporated into carnitine; however, radioactivity from dl-[1-(14)C]lysine and dl-[2-(14)C]lysine was not incorporated. Administered l-[Me-(14)C]methionine labelled only the 4-N-...

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1,1,3-Trimethyl-3-phenyl­indane

In the title compound, C(18)H(20), the five-membered ring of the indane fragment adopts an envelope conformation, with the flap atom deviating by 0.399 (3) Å from the plane of the remaining four atoms. The dihedral angle between the phenyl ring and the indane benzene ring is 79.58 (7)°.

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Role of Lysine and s-NTrimethyllysine in Carnitine Biosynthesis

We have previously reported that weanling rats fed a 20% wheat gluten diet, limiting in lysine and containing no detectable carnitine, have significantly lower levels of carnitine in skeletal and heart muscle than rats receiving adequate dietary lysine. Rats on 20% wheat gluten diets were administered appropriate levels of DL-[6-i*C]lysine, L-[mefhyZ3H]methionine, t-N-[mefhyZ-3H]trimethyl-L-lys...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1980

ISSN: 0306-3283

DOI: 10.1042/bj1880529